Journal: Phytomedicine

Article Title: Discovery of Camellia sinensis catechins as SARS-CoV-2 3CL protease inhibitors through molecular docking, intra and extra cellular assays

doi: 10.1016/j.phymed.2021.153853

Figure Lengend Snippet: Sensorgrams for (-)-epicatechin 3- O -caffeoate ( 1 ) (0.78-12.50 µM), etc-pyrrolidinone C and D ( 6 ) (0.78-12.50 μM), (+)-gallocatechin gallate (GCG) (0.39-6.25 μM), (-)-epigallocatechin gallate (EGCG as the positive control) (0.39-6.25 μM) flowing over a CM7 3CL protease-immobilized sensor-chip surface at 25°C. Steady-state affinity analysis of (-)-epicatechin 3- O -caffeoate ( 1 ), etc-pyrrolidinone C and D ( 6 ), and (+)-gallocatechin gallate (GCG) binding to 3CL protease was fitted to a 1:1 interaction model.

Article Snippet: CM7 sensor chip, 10 × PBS-P buffer (containing 0.2 M phosphate buffer, 27 mM and 1.37 M NaCl, 0.5% Surfactant P20, pH adjusted to yield pH 7.4 when diluted 10 × and supplemented with 2% DMSO), sodium acetate pH 4.0, 4.5, 5.0, 5.5 and amino coupling kit were purchased from Cytiva (Uppsala, Sweden).

Techniques: Positive Control, Binding Assay